Effects of Laminar Flow Ultraviolet Sterilization Protocol on Cryptosporidium parvum Oocysts and Determination of These Effects with Vital Dyes
نویسنده
چکیده
Cryptosporidiosis is a globally widespread protozoan disease with zoonotic character. Protection and control bear great importance for combating the disease that may cause serious economic losses. At this point, UV (ultraviolet) may be used to inactivate the oocysts those are infectious form of the disease. In this study, determination of the effect of UV sterilization process used in routine cell culture laboratory sterile cabins (laminar fl ow) on Cryptosporidium parvum oocysts and the efficiency of vital dyes (DAPI, PI) in the demonstration of the changes made by UV applications in question on oocysts is aimed.
منابع مشابه
Production of Specific Egg Yolk Antibody (IgY) against Cryptosporidium parvum Oocysts
ABSTRACT Background and Objectives: Cryptosporidium parvum is a coccidian protozoan that causes diarrhea in immunocompromised humans and newborn animals. The severity of the disease depends on the immunological status of the affected. Cryptosporiosis can have lethal effects on immunocompromised individuals such as AIDS patients. About 10% of...
متن کاملSurvival of Cryptosporidium parvum oocysts under various environmental pressures.
The survival of various isolates of Cryptosporidium parvum oocysts under a range of environmental pressures including freezing, desiccation, and water treatment processes and in physical environments commonly associated with oocysts such as feces and various water types was monitored. Oocyst viability was assessed by in vitro excystation and by a viability assay based on the exclusion or inclus...
متن کاملEffects of freeze-thaw events on the viability of Cryptosporidium parvum oocysts in soil.
The effects of freeze-thaw events on the inactivation of Cryptosporidium parvum oocysts in soil were examined. Oocysts were inoculated into distilled water in microcentrifuge tubes or into chambers containing soil the water content of which was maintained at 3%, 43%, or 78% of the container capacity. The chambers and tubes were then embedded in 3 soil samples from different aspects of a hillsid...
متن کاملA flow cytometric protocol for detection of Cryptosporidium spp.
Cryptosporidium parvum is transmitted through water and can cause severe diarrhea. The diagnosis is usually based upon observer-dependent microscopic detection of oocysts, with rather low sensitivity and specificity. Our objective was to optimize a flow cytometric (FC) protocol for the detection of C. parvum. A specific monoclonal antibody conjugated with R-phycoerythrin was incubated with dead...
متن کاملBatch solar disinfection inactivates oocysts of Cryptosporidium parvum and cysts of Giardia muris in drinking water.
AIM To determine whether batch solar disinfection (SODIS) can be used to inactivate oocysts of Cryptosporidium parvum and cysts of Giardia muris in experimentally contaminated water. METHODS AND RESULTS Suspensions of oocysts and cysts were exposed to simulated global solar irradiation of 830 W m(-2) for different exposure times at a constant temperature of 40 degrees C. Infectivity tests wer...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2010